Information on the gut microbiota testing service

The gut microbiota testing service

Gut microbiota contribute to the metabolomics profiling of organs, protection against pathogens, and development of the immune system of the host.
Consequently, the diversity of gut microbiota greatly affects the physiological function of the host's gut.
Therefore, to generate reproducible and optimal experimental results, it is important to use animals with stable gut microbiota and to monitor any changes in the microbiota.
Our center offers a gut microbiota testing service to routinely monitor the gut microbiota of mice and rats in breeding and to rapidly test experimental effects on the gut microbiota.

Service features

We use the terminal restriction fragment length polymorphism (T-RFLP) method, which we developed in collaboration with TechnoSuruga Laboratory Co., Ltd. using data of gut microbiota in mice and rats. The T-RFLP method for mice and rats provides the following features:

- Is simple, highly sensitive, and reproducible
- Provides comprehensive analysis of microbiota possible from
   one fecal sample
- Allows for estimate of microbes group using our database of
   mice and rats gut microbiota

Service content

- Enables estimate of the composition and relative ratios of gut microbiota using
- Gut microenvironment analysis (Optional items)

♦ Fecal organic acids analysis
   Succinic acid, Lactic acid, Formic acid, Acetic acid, Propionic acid, Isobutyric acid,
   n-Butyric acid, Isovaleric acid, n-Valeric acid
♦ Decomposed fecal material analysis
   Indol, Skatole, Phenol, p-Cresol
♦ Fecal ammonia concentration measurement
♦ Fecal pH measurement
♦ Quantitative PCR analysis

- Contract research services (example)